1. In addition to independent research and development of biotechnology products and technology services, the company is Comissariado MERCK (Calbiochem, Stratagene, Novagen, Novabiochem), and other well-known international biotechnology company\'s Jiangsu regional level agency, the United States Synthegen, Canada ABM companies in China distributor.
除自主研发的生物技术产品以及科技服务外，凯基公司是MERCK（Calbiochem、Stratagene、Novagen、Novabiochem）等国际著名生物技术公司的江苏地区一级代理、美国Synthegen公司、加拿大ABM公司中国地区总代理。
2. MATERIALS: Human fetal brain total RNA was purchased from the STRATAGENE Co., Ltd and reverse transcripted the cDNA libray by the Department of Pharmacology, Beijing Genomics Institute, Chinese Academy of Science.
材料：人胎脑Total RNA购自STRATAGENE公司，经中国科学院北京基因组研究所药理组反转录为cDNA文库；人胚肾细胞系HEK293由中国医学科学院基础所细胞中心提供。
3. Methods Modified phenol-chloroform method was used to isolate genomic DNA. The isolated genomic DNA was digested by Sau3AI to 40kb fragments on average. Then, the fragments were packaged by Stratagene`s Gigapack III XL packaging extract.
采用优化的酚氯仿抽提法制备DNA，并利用Sau3AI切割至平均大小为40 kb，然后使用Stratagene包装蛋白构建cosmid文库。
4. Stratagene的意思
4. Methods: 64 patients with advanced non-small cell lung cancer diagnosed by pathology and in accord with the standard of research scheme were admitted and randomized to different research scheme including different chemotherputic regimens and chemotherapy alone or chemotherapy plus traditional Chinese medicine. All patients DNA of peripheral blood leukocytes was obtained before therapy. Taqman probe were used to analyze the SNP of XRCC1 codon399 and genotypes were detected by Stratagene soft.
收集经病理学确诊并符合研究方案入选标准的晚期非小细胞肺癌64例，随机入组不同化疗方案，并随机再分单纯化疗组和化疗联合中药组；同时所有病例化疗前抽取静脉血，选用Gentra公司试剂盒提取全血基因组DNA，采用TaqMan探针法对XRCC1第399位密码子进行SNP分析，由Stratagene分析软件得出基因型。
5. Stratagene的近义词
5. But the molecular weight andsome chemical characteristics are different from the Ig proteins. We thenscreened CLONTECH HeLA S3 cDNA expression library and the HeLaMR cDNA expression library we synthesized with stratagene Uni-Zapvector by nucleotide hybridization and immuno-screen method.
在确认这些肿瘤细胞内并不存在Ig基因可变区的重排后，我们又从mRNA水平对这一问题进行了研究，我们设计了5类免疫球蛋白基因的下游引物和巢式引物，以Anchored PCR和Nested PCR相结合的方法在cDNA水平对上述肿瘤细胞中的6种细胞进行了检测。
6. Stratagene的近义词
6. METHODS: According to PCR method, gain genic sequence of NR2B C-terminal polypeptide. Insert these genic sequence into AAV-IRES-GFP vector. Using the AAV-helper free system of Stratagene company to construct recombinant AAV(AAV-GFP, AAV-33C-IRES-GFP and AAV-200C-IRES-GFP). The AAV-100-IRES- GFP and AAV-600-IRES-GFP can expression 33 or 200 amino acid of NR2B C- terminal respectively.
直接合成引物进行配对PCR或从小鼠海马基因组DNA上进行扩增获得目的基因，将获得的目的基因连接到Stratagene公司的AAV-IRES-GFP质粒上，使用AAV Helpe-Free system来构建能表达NR2B羧基端33个和200个氨基酸多肽的重组AAV病毒(AAV-33C-IRES-GFP and AAV-200C-IRES-GFP)，应用重组病毒感染原代神经元，观察其是否能阻断由谷氨酸引起的神经元损伤。
7. METHODS: Mammalian Two-Hybrid Assay Kit produced by Stratagene Co. Ltd was adopted in the present research. The plasmid pCMV-BD-JTB, pCMV-AD-S and report plasmid pFR-Luc were co-transferred into 293FT cell line to evaluate the interaction between JTB protein and HBsAg.
目的：本研究采用哺乳动物细胞双杂交、免疫共沉淀以及GST pull-down等方法进一步确认HBV包膜蛋白与跳跃断裂易位(Jumping Translocation Breakpoint，JTB)蛋白之间的相互作用。
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